EI 1352 ANALYTICAL INSTRUMENTS 3 0 0 100
AIMThe course is designed to equip the students with an adequate knowledge of a number of analytical tools which are useful for clinical analysis in hospitals, drugs and pharmaceutical laboratories and above all for environmental pollution monitoring and control.
OBJECTIVES
i. To provide various techniques and methods of analysis which occur in the various regions of the spectrum. These are the powerful tools used in clinical and research laboratories.
ii. To give unique methods of separation of closely similar materials, the most powerful being gas chromatography.
iii. To study important methods of analysis of industrial gases. Awareness and
control of pollution in the environment is of vital importance.
iv. To bring out the latest ideas on ion-selective electrodes as well as biosensors which have potential applications in medical field, food and beverage industries.
v. To provide the important radio chemical methods of analysis. Further they are both sensitive and specific and often are characterized by good accuracy. NMR & ESR techniques are useful in structure determination.
1. COLORIMETRY AND SPECTROPHOTOMETRY 10
Special methods of analysis – Beer-Lambert law – Colorimeters – UV-Vis spectrophotometers – Single and double beam instruments – Sources and detectors – IR
spectrophotometers – Types – Attenuated total reflectance flame photometers – Atomic absorption spectrophotometers – Sources and detectors – FTIR spectrophotometers – Flame emission photometers.
2. CHROMATOGRAPHY 8
Different techniques – Gas chromatography – Detectors – Liquid chromatographs – Applications – High-pressure liquid chromatographs – Applications.
3. INDUSTRIAL GAS ANALYZERS AND POLLUTION MONITORING
INSTRUMENTS 9
Types of gas analyzers – Oxygen, NO2 and H2S types, IR analyzers, thermal conductivity analyzers, analysis based on ionization of gases. Air pollution due to carbon monoxide, hydrocarbons, nitrogen oxides, sulphur dioxide estimation - Dust and smoke measurements.
4. pH METERS AND DISSOLVED COMPONENT ANALYZERS 8
Principle of pH measurement, glass electrodes, hydrogen electrodes, reference electrodes, selective ion electrodes, ammonia electrodes, biosensors, dissolved oxygen analyzer – Sodium analyzer – Silicon analyzer.
5. RADIO CHEMICAL AND MAGNETIC RESONANCE TECHNIQUES 10
Nuclear radiations – Detectors – GM counter – Proportional counter – Solid state detectors – Gamma cameras – X-ray spectroscopy – Detectors – Diffractometers – Absorption meters – Detectors. NMR – Basic principles – NMR spectrometer - Applications. Mass spectrometers – Different types – Applications.
L = 45 Total = 45
TEXT BOOKS
1. R.S. Khandpur, ‘Handbook of Analytical Instruments’, Tata McGraw Hill publishing Co. Ltd., 2003.
2. H.H.Willard, L.L.Merritt, J.A.Dean, F.A.Settle, ‘Instrumental methods of analysis’, CBS publishing & distribution, 1995.
REFERENCE BOOKS
1. Robert D. Braun, ‘Introduction to Instrumental Analysis’, McGraw Hill, Singapore, 1987.
2. G.W.Ewing, ‘Instrumental Methods of Analysis’, McGraw Hill, 1992.
3. DA Skoog and D.M.West, ‘Principles of Instrumental Analysis’, Holt, Saunders Publishing, 1985.
4. C.K. Mann, T.J Vickers & W.H. Gullick, ‘Instrumental Analysis’, Harper and Row publishers, 1974.