BM1258 Pathology and Microbiology Lab Syllabus

BM1258 PATHOLOGY AND MICROBIOLOGY LAB 0 0 4 100

1. Haemoglobin Estimation.
2. Peripheral Smear Study.
3. Urine Smear Study
4. Cross matching of Blood.
5. Tissue Biopsy – Benign and Malignant.
6. Simple Stain test
7. Gram Stain test.
8. AFB Stain test.

List of equipment for a batch of 30

Sl.No
Description
Quantity
required
1.
Haemoglobin Estimation
Sahil’s Haemoglobinometer

1 set
2.
Peripheral Smear Study
Peripheral smear – Routine Leishman’s Stain
RBC – Size, Shape Inclusions in RBC
WBC – Differential count
Glass Slide, Binocular Microscope with light


1 set
3.
Urine Smear Study
Urine Smear, Glass Slide, Cover slips Binocular Microscope with light

1 set
4.
Cross matching of Blood
Blood Grouping : cross – matching with respective centisera available in the market Slide, Antisera, Binocular Microscope with light

1 set
5.
Tissue Biospy – Benign and Malignant
Tissue Biospy – Aspirating biopsy needle (under doctor’s supervision) Glass slide

1 set
6.
Simple Stain test
SIMPLE STAIN, Glass Slide
Reagents – Methylene blue, Crystal Violet, Cabal Iuschin

1 set
7.
Gram Stain test
SIMPLE GRAM STAIN
      Reagents – Crystal Violet, Gram’s Iodine, 95% ethyl       alcohol Safranin
      Urine bacterial count/ml exceeding 100,000(10s)  denotes urinary tract infection.
     Normal : 0-100 ml
       Glass Slide


1 set
8.
AFB Strain test
ACID FAST STAIN (ZIEHL – NEELSON METHOD)
Reagent – Carbal Iushin, Acid Alcohol, Methylene blue, Glass slide

1 set
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