BM1204 BioChemistry and Human Physiology Lab Syllabus

BM1204 BIO CHEMISTRY AND HUMAN PHYSIOLOGY LAB 0 0 4 100

1. Recording of Muscle Response to Induced Electrical Stimulation
2. Study of rate of Conduction of Nerve Impulses.
3. Isolated Frog Heart Perfusion and Effect of ionic Changes.
4. Testing of Hearing using Tuning Fork.
5. Testing of various parameters of Vision and Errors of Refraction.
6. Testing for Detection of Glucose, Fructose and Starch.
7. General Test for Proteins
8. Testing of Urine for presence of Sugar, Protein
9. Estimation using Spectrophotometer

List of equipment for a batch of 30

Sl.No
Description
Quantity
required
1.
Recording of Muscle Response to induced Electrical Stimulation
(i)     Low voltage (D.C)unit                                                                                                        
(ii)   DuBois Raymond induction coil with Neef’s   hammer – to obtain alternate faradic current
(iii)  Vibrating Reed
(iv)   Stimulating electrodes
(v)    Tap key
(vi)   Tuning fork (100 Hz)


1 No
2 Nos

2 Nos
5 Nos
5 Nos
2 Nos
2.
Study of rate of Conduction of Nerve Impulses
(i)     Sherington – Starling drum
(ii)    Lucas trough / Muscle bath
(iii)   Writing lever with after load screw (Isotonic   Muscle Lever)
(iv)   Electrical Kymograph
(v)     Muscle bath stand and S clamps Hook and 
         weight

2 Nos
2 Nos
4 Nos

2 Nos
2 Nos
3.
Isolated Frog Heart perfusion and Effect of ionic changes
(i)     Mariotte’s bottle (Perfusion bottle)
(ii)   Rubber tubing
(iii)  Syme’s Cannula
(iv)  Frontal lever/ Starling’s Heart lever                                              
(v)   Myograph board



1 set
4.
Testing of Hearing using Tuning Fork
Tuning Fork with 256 Hz
2 Nos
5.
Testing of various parameters of Vision and Errors of Refraction
Color Blindness – Isihara chart
Visual acuity – Snellen’s chart
Myopia + Hyperopia – by letters reading
Ophthalmoscope – to view retina


1set

6.
Testing for Detection of Glucose, Fructose and Starch
(i) Test for detection of Glucose
      Source – Blood
      Estimation – Spectrophotometer
      Reagent : Alkaline Cupric Tartarate
      Solution Phosphomolybdic acid
(ii) Test for detection of Fructose
      Reagent : 1% alpha napthol Concentrated
      Hydrochloric acid
(iii) Test for detection of starch
       Reagent : Iodine
(iv) Test tube






1 set
7.
General Test for Proteins
General test for Proteins
Reagent : Concentrated Nitric acid

1 set
8.
Testing of Urine for Presence of Sugar, Protein
Testing of Urine for Presence of Sugar, Protein of
  Sugar Reagent – Benedict’s reagent
 Protein Reagent – 1% Acetic acid 

1 set
9.
Estimation using Spectrophotometer
 Spectrophotometer

1 No

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