BM1203 BioChemistry Syllabus

BM1203 BIOCHEMISTRY 3 0 0 100

AIM
• To give a clear picture about various chemical activities taking place at cellular level.
• To understand the various chemical activities taking place in blood, muscles and at neuromuscular junctions.

OBJECTIVES
• To study the biochemical activities taking place at cellular level.
• To study the chemical composition of blood and urine in normal and abnormal conditions.
• Chemical activities taking place at muscles and neuromuscular
junction.
• To study the various analysis to be done in the biofluids and different equipments used for this purpose.

1.UNIT-I 9
Biochemistry of living cell, Sub cellular fractionation using the differential centrifugation method. Function of each organelle Redox Potential, Oxidative Phosphorylation , Transport of substances across biological membrane.
NUCLEIC ACID: Composition and Function, Genes, Outline of DNA Structure,
Re-Combinant DNA and its applications.

2. ENZYMES AND HORMONES 9
Enzymes: Chemical Nature, General Properties, Spectrophotometric measurement of enzymes, Isolation techniques, Diagnostic enzymes. Enzyme biotechnology.
Hormones: Chemical Nature, Properties of hormones, Hormonal Assay and their Significance.

3. CARBOHYDRATE, LIPID, PROTEIN 9
(i) Carbohydrate – Classification, Metabolism of carbohydrate and its dysfunction. Uses of Carbohydrates.
(ii) Lipids: Classification, Metabolism of lipids, Cholesterol, bile acids, Transport of lipids, Lipid metabolism dysfunction.
(iii) Protein: Classification, Amino acids, Chromatography, electrophoresis and architecture of protein molecules.

4.UNIT-IV 9
(i) BIO CHEMISTRY OF BLOOD AND BODY FLUIDS: Liver Function tests, Renal Function Tests, Blood gas Analysis, Measurement of Electrolytes. Their abnormal and Normal Values and Conditions.
(ii) Biochemistry of Urine and Stools testing.

5. DIAGNOSTIC TOOL 9
Principles and Application of Photometry , Spectrophotometry, Flurometry, Flame Photometry, Densitometry, Calorimetry, Automation in Clinical Laboratory. Use of Isotopes in Biochemistry.

TOTAL : 45

TEXT BOOK
1. Dr. Ambiga Shanmugam, ‘Fundamentals of Bio Chemistry for Medical Students’, Karthic Printers, Madras 1997.
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